Year 12 Human Biology

Chromatography

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A technique used to separate a mixture into different substances.

Below you can see a chromatogram:

  1. Draw the origin line in pencil (starting point)
  2. The solution is spotted onto the origin
  3. Paper is suspended in a tank of solvent and left
  4. The final place where the solvent reaches is marked the solvent front

chromatog

Identifying the mixture:

  1. Measure the distance from the origin to the middle of the spot
  2. Measure the distance from the origin to the solvent front
  3. We identify the spots using an Rf value, Rf = distance travelled by spot/distance travelled by solvent
  4. Then compare your value to one in the table – Rf values are always less than 1!!!! If it is higher you have done something wrong!!!!
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One thought on “Chromatography

  1. Pingback: Colonization, Size Exclusion Chromatography | NerdlyPainter

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